Latest News on Bacteriological Quality Research: Dec – 2019

Bacteriological Quality of Runoff Water from Pastureland

Runoff from a cow-calf pasture in eastern Nebraska was monitored for total coliforms (TC), fecal coliforms (FC), and fecal streptococci (FS) during 1976, 1977, and 1978. Bacteriological counts in runoff from both grazed and ungrazed areas generally exceeded recommended water quality standards. The FC group was the simplest indicator group of the impact of grazing. Rainfall runoff from the grazed area contained 5 to 10 times more FC than runoff from the fenced, ungrazed area. There was little difference in TC counts between the 2 areas, but FS counts were higher in runoff from the ungrazed area and reflected the contributions from wildlife. Recommended bacteriological water quality standards, developed for beginning inputs, could also be inappropriate for characterizing nonpoint source pollution from pasture runoff. [1]

Bacteriological quality and safety of raw milk in Malaysia

The microbiological safety of milk from 360 dairy farms in Peninsular Malaysia decided. Milk samples were collected at 40 Milk Collection Centers (MCC) from four regions, namely, Southern (Johor/Melaka), Central (Selangor/Negeri Sembilan), Northern (Perak/Kedah) and Eastern (Kelantan/Terengganu) consistent with stratified sampling design. Samples were analyzed for Total Plate Count (TPC), Staphylococcus aureus, coliform and Escherichia coli also because the prevalence of selected pathogens like Listeria monocytogenes, E. coli 015:H7 and Salmonella. The mean counts per ml for TPC, psychrotrophs and thermophiles were 12×106, 7.5×103 and 9.1×103, respectively. A TPC but 106 cfu ml−1 is employed as a basic standard by MCC within the Price Incentive Programme. From the 930 milk samples tested, approximately 90% were contaminated by coliform bacteria and 65% were E. [2]

Escherichia coli as an indicator of bacteriological quality of water: an overview

Monitoring the microbiological quality of beverage relies largely on examination of indicator bacteria like coliforms, Escherichia coli, and Pseudomonas aeruginosa. E. coli may be a member of the faecal coliform group and may be a more specific indicator of faecal pollution than other faecal coliforms. Two key factors have led to the trend toward the utilization of E. coli because the preferred indicator for the detection of faecal contamination, not only in beverage, but also in other matrices as well: first, the finding that some faecal coliforms were non faecal in origin, and second, the event of improved testing methods for E. coli. The faecal coliform definition has also been revised to coincide better with the genetic make-up of its members and now includes newly identified environmental species. [3]

Bacterial Quality Control in Human Milk Banking

We have analysed the bacteriological quality of milk donated to the Oxford milk bank: we’ve studied the consequences on bacteriology of straightforward additional antisepsis during the gathering procedure and of Holder pasteurisation during a purpose built human milk pasteuriser. 12 pools of human drip breast milk (each comprising 75-120 24 hour milk samples) were studied: 6 pools were collected into vessels washed in detergent (Group I): within the other 6 a hypochlorite sterilising agent was used (Group II). Potentially pathogenic organisms were grown in untreated milk from both group I & II pools and included E.coli, S.aureus and B B-haemolytic streptococci. 7 species of non-pathogenic organisms were identified from both group I & II pools. Pasteurisation eliminated all potential pathogens from both groups, but didn’t reliably remove any of the non-pathogenic species. [4]

Bacteriological Quality Assessment of Bottled Water Brands Marketed in Kitale Town, Trans- Nzoia County, Kenya

Background: Consumption of drinking water is increasing rapidly in developing countries because it is usually seemed to be pure, clean and of excellent quality. This has led to the sale of various brands of drinking water in several markets including Kitale town.

Aim of the Study: This study was conducted to assess the bacteriological quality of drinking water brands consumed in Kitale town.

Study Design: it had been a cross-sectional study design which involved getting a snap shot situation of the prevailing bacteriological standards of drinking water sold within the Kitale town area.

Place and Duration of the Study: the present study was conducted in Kitale town area among outlets of drinking water brands. [5]


[1] Doran, J.W. and Linn, D.M., 1979. Bacteriological quality of runoff water from pastureland. Appl. Environ. Microbiol., 37(5), (Web Link)

[2] Chye, F.Y., Abdullah, A. and Ayob, M.K., 2004. Bacteriological quality and safety of raw milk in Malaysia. Food microbiology, 21(5), (Web Link)

[3] Odonkor, S.T. and Ampofo, J.K., 2013. Escherichia coli as an indicator of bacteriological quality of water: an overview. Microbiology research, 4(1), (Web Link)

[4] Bacterial Quality Control in Human Milk Banking
A Lucas, C Roberts & J D Baum
Pediatric Research volume 13, (Web Link)

[5] Y. Adaro, I., G. James, N. and Ndung’u, P. (2017) “Bacteriological Quality Assessment of Bottled Water Brands Marketed in Kitale Town, Trans- Nzoia County, Kenya”, Journal of Advances in Microbiology, 2(4), (Web Link)

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