The Use of Spectrophotometric and Thermodynamic Methods for the Determination of Glibenclamide

For the assay of glibenclamide, a spectrophotometric and thermodynamic method is defined. The technique involves a glibenclamide charge transfer complexion reaction with 2,3-Dichloro-5,6-Dicyano-1,4-benzoquinone (DDQ) to form a very intense coloured charge transfer complex with a 1:1 stoichiometric ratio. Under tailored experimental conditions, the resulting coloured product was analysed at 474 nm. Beer-Lambert ‘s law was obeyed over the 20-100 μg / ml concentration range. The apparent absorptivity of the molar was measured and 0.99 ⁇ 103 L.mol- 1 cm-1 was found. The corresponding sensitivity of Sandell, detection limits, and quantification were stated. The thermodynamic parameters have been defined as well. The proposed method was applied successfully to the determination of glibenclamide in pure and commercial forms with strong average recovery of 98.24 percent. The student t-test and f-test were used for statistical comparison at a 95 percent confidence level.

Author (s) Details

Nwanisobi Gloria
Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka, Nigeria.

Ukoha Pius
Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, University of Nigeria, Nsukka, Nigeria.

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