Antibiotic Resistance Profile Studies of Bacteria from water in Excavated Ponds in Port Harcourt Metropolis, Nigeria

In the metropolis of Port Harcourt , Rivers State , Nigeria, the Antibiotic Resistance profile of bacteria isolated from water from two excavated (undeveloped and developed) ponds was investigated using standard microbiological techniques. In a recreational facility called the Pleasure Park, the created pond is The undeveloped pond is situated with minimal facilities in a densely populated built-up area. The following bacterial species were found in water samples from the undeveloped pond: Escherichia coli, Staphylococcus spp.; Shigella spp.; Klebsiella spp.; Vibrio spp. Salmonella spp. and The overall culturable heterotrophic bacterial count (TCHB) and faecal coliform count ranged from 1.02 x 106-1.90 x 106cfu / ml and 3.70 x 105-8.15 x 105cfu / ml respectively in the water samples from the undeveloped pond. The bacteriological (TCHB) load of water samples ranged from 6.9 x 105cfu / ml to 2.1 x 106cfu / ml from the formed pond, while the faecal coliform count ranged from 3.4 x 105 to 1.3 x x coliform count From 106cfu / ml. Escherichia coli, Salmonella spp., Proteus spp., Citrobacter spp., Klebsiella spp., Bacillus spp., Serratia spp., Pseudomonas spp., Vibrio spp., Enterobacter spp., Staphylococcus aureus, Streptococcus spp., and Staphylococcus spp. were bacterial species found in an established pond. Using the Kirby-Bauer disc diffusion process, the sensitivity of the isolates from the water samples to 12 different antibiotics chosen was developed on Muller-Hinton agar. The zone diameter obtained was interpreted using the zone diameter breakpoints of the Clinical Laboratory Standard International (CLSI) and the British Society for Antimicrobial Chemotherapy (BSAC). The degree of resistance to various antibiotics used was shown by the isolates from the undeveloped pond; Lincocin 74.8%, Rifampicin 71.4%, Augumentin 71.2%, Chloramphnicol 68.2%, Erythromycin 64.3%, Cotrimoxazole 55.8%, Streptomycin 50.2%, Pefloxacin 48.6%, Gentamycin 43%, Norfloxacin 42.9%, Ofloxacin 16.2%,Pond, uh. Many of the bacteria isolated are potential pathogens. Isolates from the produced pond showed varying levels of resistance to 14 tested antibiotics, with the highest resistance to Pefloxacin (55.9 percent) while the lowest resistance to Erythromycin (2.9 percent). A 38.2 percent multidrug resistance rate was shown by an MDR (resistance to 3 or more antibiotic classes) assessment. Conceptual risk models (CRM) and drug resistance mapping are critical in addressing the occurrence of drug resistance in target populations as part of health management in developing countries.

Author (s) Details

Professor G. O. Abu
Department of Microbiology, University of Port Harcourt, PMB 5323, Port Harcourt, Nigeria.

A. C. Wondikom
Department of Microbiology, University of Port Harcourt, PMB 5323, Port Harcourt, Nigeria.

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