More than half of the cancer patients undergoing radiotherapy have received radiotherapy during the course of care, and it is the most common advanced-stage treatment for cervical cancer. The goal of this study is to identify changes in gene expression involving gamma radiation-induced apoptosis pathways, which identified genes and gene-related signalling pathways may provide meaningful biomarkers for understanding cancer dynamics and human cervical cancer treatment targets. Various doses of a single fraction of gamma radiation were exposed to cervical cancer cells. After incubation for different times, the MTT assay examined the proliferation of C-4 I and HeLa cells, whereas morphological characteristics were evaluated by fluorescent microscopy to measure the Apoptotic Index (AI). In addition, gene expression was assessed using molecular micro-array processes and study of the signalling pathway was carried out. Gamma irradiation inhibits HeLa and C-4 I cell proliferation in a time- and dose-dependent manner. A substantial difference between HeLa and C-4 I cell lines was observed from our observations (p<0.01), while HeLa cells tended to be radio resistant, while C-4 I cells were radiosensitive. The doses of IC50 and AI were 16 Gy and 32 Gy for C-4 I and HeLa cells, respectively. The results of the microarray controlled the expression of some factors considered to be gamma radiation treatment-regulated apoptosis activators, whereas some representatives of anti-apoptosis were down-regulated. Analysis of the pathway identified that essential pathways related to apoptosis, WNT, cell cycle and P53 were significantly improved. These findings indicate that ionised radiation directly induces anti-proliferative effects by transforming the expression in HeLa and C-4 I cervical cancer cells of genes linked to apoptosis and cell proliferation pathways. Specific gene identification can be helpful in a novel treatment strategy to improve the sensitivity of cancer cells to radiotherapy by modulating the expression of several genes. Instead of comprehensive research, our analysis is a sort of screening. In order to identify these defined genes in vitro and in vivo, we need further study.
Walid M. Khalilia
Department of Forensic Sciences, Al-Istiqlal University, Jericho, Palestine.
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