Induction of Apoptosis Pathways in Human Cervical Carcinoma Cells by Ionizing Radiation

More than half of the cancer patients undergoing radiotherapy have received radiotherapy during the course of care, and it is the most common advanced-stage treatment for cervical cancer. The goal of this study is to identify changes in gene expression involving gamma radiation-induced apoptosis pathways, which identified genes and gene-related signalling pathways may provide meaningful biomarkers for understanding cancer dynamics and human cervical cancer treatment targets. Various doses of a single fraction of gamma radiation were exposed to cervical cancer cells. After incubation for different times, the MTT assay examined the proliferation of C-4 I and HeLa cells, whereas morphological characteristics were evaluated by fluorescent microscopy to measure the Apoptotic Index (AI). In addition, gene expression was assessed using molecular micro-array processes and study of the signalling pathway was carried out. Gamma irradiation inhibits HeLa and C-4 I cell proliferation in a time- and dose-dependent manner. A substantial difference between HeLa and C-4 I cell lines was observed from our observations (p<0.01), while HeLa cells tended to be radio resistant, while C-4 I cells were radiosensitive. The doses of IC50 and AI were 16 Gy and 32 Gy for C-4 I and HeLa cells, respectively. The results of the microarray controlled the expression of some factors considered to be gamma radiation treatment-regulated apoptosis activators, whereas some representatives of anti-apoptosis were down-regulated. Analysis of the pathway identified that essential pathways related to apoptosis, WNT, cell cycle and P53 were significantly improved. These findings indicate that ionised radiation directly induces anti-proliferative effects by transforming the expression in HeLa and C-4 I cervical cancer cells of genes linked to apoptosis and cell proliferation pathways. Specific gene identification can be helpful in a novel treatment strategy to improve the sensitivity of cancer cells to radiotherapy by modulating the expression of several genes. Instead of comprehensive research, our analysis is a sort of screening. In order to identify these defined genes in vitro and in vivo, we need further study.

Author(s) Details

Walid M. Khalilia
Department of Forensic Sciences, Al-Istiqlal University, Jericho, Palestine.

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