Effects of CTHepaB, a Traditional Vietnamese Medicinal Formulation, on the Treatment of Cirrhosis in Wistar Rats

Objectives: CTHepaB is a popular Vietnamese herbal formulation that is used to avoid liver problems. In this analysis, the effects of CTHepaB on cirrhosis in Wistar rats caused by carbon tetrachloride (CCl4) combined with alcohol, high-fat, and iron diet were evaluated in vivo.

Materials and Methods : Cirrhosis was induced in Wistar rats by injecting CC14 (5.0 ml/kg) subcutaneously once a week for 10 weeks. The rats were then given a diet that included 20% fat, 0.05 percent cholesterol, and iron oxalate. Every day, rats were given plain water, and on one day, they were given water laced with 30% ethanol. The rats were divided into five groups and given distilled water, CC14, silymarin (70 mg/kg/24 h), or CTHepaB (at 0.56 and 1.12 g/kg/24 h) for four weeks, during which blood samples were taken for biochemical studies, the livers were dissected to assess weight and morphology, and 4-hydroxyproline was quantified to assess fibrosis and collagen accumulation.

Results : CTHepaB reduced the activity of the enzymes aspartate transaminase (AST) and alanine transaminase (ALT) in the blood in cirrhotic Wistar rats at both 0.56 and 1.12 g/kg/24 h (p 0.01), increased plasma albumin levels, and decreased prothrombin times (p 0.05). According to Masson’s trichrome-stained templates, CTHepaB treatment strengthened the rats’ physical condition, decreased hydroxyproline concentrations in the liver, and minimised the level of cirrhosis. CTHepaB had dose-dependent effects, with a dose of 70 mg/kg/24 h being equivalent to silymarin.

Conclusion: CTHepaB was effective for the treatment of a cirrhosis model in Wistar rats.

Author (s) Details

Dau Xuan Canh
Vietnam University of Traditional Medicine, 2 Tran Phu, Mo Lao Ward, Ha Dong district, Hanoi, Viet Nam.

Le Thi Tuyet
Vietnam University of Traditional Medicine, 2 Tran Phu, Mo Lao Ward, Ha Dong district, Hanoi, Viet Nam.

Manh Hung Tran
Faculty of Pharmacy, Dong A University, 33 Xo Viet Nghe Tinh, Hoa Cuong Nam ward, Hai Chau District, Da Nang City 550000, Vietnam.

Nguyen Hoang Ngan
Vietnam Military Medical University, 160 Phung Hung, Phuc La ward, Ha Dong district, Hanoi, Viet Nam.

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