Recent Advances in the Use of the SNAP-tag® in the Modern Biotechnology

AGTs (alkylguanine-DNA-alkyltransferases) play a normal function in protecting DNA from alkylating agent-induced mutations. Their unique irreversible self-alkylation reaction has contributed to the creation of new biotechnology techniques. SNAP-tag® is an effective enzyme that uses benzyl-guanine (BG) derivatives as substrates to specifically mark proteins and enzymes. The mesophilic nature of the tag (an engineered version of the human enzyme) and the need to purify each substrate are two drawbacks of this technology. The use of thermostable “SNAP-tag-like” variants from (hyper)thermophilic sources, as well as BG-substrates containing an azide group to be combined with DBCO-derivatives by azide-alkyne Huisgen cycloaddition, has recently been active in implementing the SNAP-tag® technology. With the addition of these new players to the equation, the technique could be expanded to include in vivo and in vitro harsh reaction conditions, as well as the use of more chemical groups in the overall reaction enzyme labelling.

Author (s) Details

Rosa Merlo
Institute of Biosciences and BioResources, National Research Council of Italy, Via Pietro Castellino 111, 80131 Naples, Italy.

Rosanna Mattossovich
Institute of Biosciences and BioResources, National Research Council of Italy, Via Pietro Castellino 111, 80131 Naples, Italy.

Anna Valenti
Institute of Biosciences and BioResources, National Research Council of Italy, Via Pietro Castellino 111, 80131 Naples, Italy.

Giuseppe Perugino
Institute of Biosciences and BioResources, National Research Council of Italy, Via Pietro Castellino 111, 80131 Naples, Italy.

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