Research on Semiquantification of Lethal Toxin at the Bottom of Reactors for Clostridium sordellii Culture

The veterinary vaccine for Clostridium sordelli is created by cultivating the microorganism in large industrial bioreactors and purifying the toxin from the culture supernatants. The harvesting time is currently determined by bacterial growth, but this time is not always correlated with the highest concentration of toxin in the supernatant. As a consequence, determining the total amount of toxin released from batch to batch is easy. Furthermore, the toxin concentration is currently determined using in vivo LD50 methods, with findings available only 72 hours after the bioreactor has been turned off. Furthermore, the procedure is time-consuming and requires the use of a large number of test animals. The development and use of a latex agglutination reagent for semi-quantification of Clostridium sordelli lethal toxin (TcsL) in industrial bioreactors is identified. The reagent was developed and tested in our lab, with a detection limit of 8ng of toxin per ml of culture supernatant, before being validated in real-world conditions. The use of such a fast (i.e., in minutes) and easy reagent would allow for real-time monitoring of the culture, standardising the best end-point for toxin quantity harvesting. As a result, TcsL industrial output quality could be increased right away.

Author (s) Details

Diana Pérez-Etcheverry
Laboratorio de Biotecnología, Instituto Polo Tecnológico de Pando-Facultad de Química, Universidad de la República, Uruguay.

Alberto Nieto-Cadenazzi
Laboratorio de Biotecnología, Instituto Polo Tecnológico de Pando-Facultad de Química, Universidad de la República, Uruguay.

Iris Miraballes-Martínez
Laboratorio de Biotecnología, Instituto Polo Tecnológico de Pando-Facultad de Química, Universidad de la República, Uruguay.

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