The aim of this study was to see how imidacloprid affected biochemical parameters and soil isolate development. The degradation of imidacloprid in the soil isolate was also investigated.
The soil isolate was identified and used for toxicity testing in this study. The capacity of Bacillus weihenstephanensis to degrade imidacloprid in minimal salt medium (MSM) and tryptic soya medium was also tested (TSB). Curing experiments were used to determine the function of plasmid in imidacloprid degradation.
Between June 2011 and December 2012, I studied at Karnatak University’s Department of Biotechnology and Microbiology in Dharwad, India.
The following is the methodology: Morphological, biochemical, and 16s rDNA characters were used to identify the soil isolate. With 10-3 to 10-7 molar imidacloprid, the effect of imidacloprid on DNA, RNA, protein, glucose, and growth in soil isolates was studied for 96 hours. Imidacloprid degradation in MSM and TSB was studied for 28 days, with samples taken on days 7, 14, 21, and 28. HPLC was used to determine the insecticide concentration. It was decided to cure the plasmids.
Bacillus weihenstephanensis was described as the soil isolate. The use of 10-3 to 10-7 molar imidacloprid on the soil isolate Bacillus weihenstephanensis resulted in a substantial (P0.05) decrease in DNA, RNA, protein, glucose, and development. In four weeks, Bacillus weihenstephanensis in MSM and TSB degraded imidacloprid by 46 and 78 percent, respectively. Bacillus weihenstephanensis plasmid was cured in the fourth century. In TSB, cured and non-cured Bacillus weihenstephanensis cells showed 18.80% and 75% degradation, respectively.
Conclusion: Imidacloprid has an impact on the biochemical content and internal growth of Bacillus weihenstephanensis, a soil isolate. Bacillus weihenstephanensis was also found to be capable of degrading imidacloprid in MSM and TSB. Further plasmid curing showed that imidacloprid degradation genes are found on both the plasmid and the chromosome. As shown by its growth in MSM, the soil isolate was able to use imidacloprid as its sole carbon and nitrogen source.
Author (s) Details
Department of Botany, GMS Academy First Grade College, P. B. Road, Davangere, India.
B. B. Kaliwal
Department of Computer Application, Visvesvaraya Technological University, P. G. Center Gulbarga, India.
R. B. Kaliwal
Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India.
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