Changes in Gut Microorganism in Patients with Positive Immune Antibodies Associated Recurrent Abortion: An Advanced Study

The goal of this study was to use a 16s rRNA gene sequencing microbiome assay to examine alterations in the gut microbiota of individuals with positive immunological antibody-associated recurrent abortion.

Methods: Fecal samples were taken from 20 recurrent abortion women with positive immune antibodies (positive group) and 20 recurrent abortion women with negative immune antibodies (negative group). Following 16s rRNA gene sequencing, raw reads were quality filtered to obtain clean tags, which were then categorised into microbial genomes. All effective tags were grouped into operational taxonomic units (OTUs), and a sample sequence was chosen for taxonomic annotation, as well as alpha and beta diversity assessments.

The total number of OTUs found in all 40 samples was 43,116. In the positive group, Bacteroides had the highest relative abundance. Bacteroides, Erysipelotrichaceae UCG-003, Faecalibacterium, and Prevotella 9 had high relative abundance in the negative group. The positive group had higher community richness, community diversity, and phylogenetic diversity than the negative group, according to the results of the Alpha diversity analysis. There were substantial differences between the negative and positive groups of Prevotella 9, Enterococcus, Megasphaera, and Anaerostipes.

Conclusion: Using a 16s rRNA gene sequencing microbiome test, the current study studied the gut microbiota involved in positive immunological antibody related recurrent abortion for the first time. The genera that differed significantly between the positive and negative groups could be used as therapeutic targets for recurrent abortion caused by positive immunological antibodies.

Author(s) Details

Min Jin
Department of Anesthesiology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China.

Xin Feng
Qilu Hospital, Shandong University, Jinan, Shandong 250012, China.

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