Impact of Alcohol Exposure on the Composition of HeLa-Derived Extracellular Vesicles

Extracellular vesicles are nanosized vesicles whose function in intercellular communication is being studied extensively. Extracellular vesicles are being studied for their potential as disease biomarkers and/or vaccine agents, as well as their function in disease defence. Since cervical cancer has such a high mortality rate, more research about how to diagnose and treat the disease is required. Several researchers have begun to look into extracellular vesicles and their function in disease defence in this regard. The aim of this study was to see how alcohol affected the biogenesis and composition of extracellular vesicles derived from the HeLa cervical cancer cell line. HeLa cells were cultured in exosome-free media and either mock-treated (control) or treated with 50 mM or 100 mM alcohol for 24 and 48 hours, respectively. Alcohol has an important effect on HeLa cell viability and exosome biogenesis/composition, according to our findings. Our findings show that alcohol has a significant impact on HeLa cells, as well as the biogenesis and composition of HeLa-derived extracellular vesicles. These findings show that alcohol affects the packaging of heat shock proteins and apoptotic proteins in extracellular vesicles. Extracellular vesicles are both communicators for HeLa cells and biomarkers for the disease’s onset and progression. The research results may have significant effects on diagnostics and therapy for a variety of cell types and organ systems, as HeLa cells were used as the primary model in this study. To elucidate the mechanism(s) involved in these processes, further research is needed.

Author (s) Details

Leandra B. Jones
Microbiology Program, Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA.

Sanjay Kumar
Department of Pediatrics and Cell, Developmental and Integrative Biology, Division of Neonatology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Aliyah J. Curry
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA and Center for Nanobiotechnology Research (CNBR), Alabama State University, Montgomery, AL 36104, USA.

Jayde S. Price
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA and Center for Nanobiotechnology Research (CNBR), Alabama State University, Montgomery, AL 36104, USA.

Alexandre Krendelchtchikov
Department of Pediatrics and Cell, Developmental and Integrative Biology, Division of Neonatology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Brennetta J. Crenshaw
Microbiology Program, Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA.

Courtnee’ R. Bell
Microbiology Program, Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA.

Sparkle D. Williams
Department of Pediatrics and Cell, Developmental and Integrative Biology, Division of Neonatology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Tambre A. Tolliver
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA.

Sabita N. Saldanha
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA.

Brian Sims
Department of Pediatrics and Cell, Developmental and Integrative Biology, Division of Neonatology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Qiana L. Matthews
Microbiology Program, Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA and Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA.

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