Coryphantha macromeris Cell Suspension Cultures: Phytochemical Profiling and Agitation Velocity Effect on Cell Morphology and Viability

For the generation of active plant metabolites, cell suspension cultures are a viable option. The agitation velocity is one of the most important factors in achieving high cell viability and metabolite yields. The effect of agitation velocity on cell viability and morphology of Coryphantha macromeris cell suspension cultures was investigated in this study, and the phytochemical profile of two-month-old cells cultivated at 80 rpm was analysed using Ultra-High-Performance Liquid Chromatography-tandem Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). The results showed that agitation at 120 rpm had a deleterious impact on cell integrity, but at 80 and 100 rpm, the cells survived and proliferated successfully with similar viability percentages (ca. 97 percent ). The presence of 49 metabolites was discovered using chromatographic and mass spectrum analysis, with 45 of them being recognised. Several classes of metabolites were discovered, including phenolic acids (gallic acid derivatives), iridoids (gardoside), stilbenes (tyrolobibenzyl E), lignans (acanthoside B), flavonoids (catechin, lantanoside, sakuranin, afrormosin, kaempferol 7-rhamnoside), and phenylethanoids (phlomisethanoside).

Author (s) Details

E. Cabañas-García
Unidad Profesional Interdisciplinaria de Ingeniería Campus Zacatecas – Instituto Politécnico Nacional. Blvd. del Bote 202 Cerro del Gato, Ejido La Escondida, Col. Ciudad Administrativa, C.P. 98160, Zacatecas, México.

C. Areche
Laboratorio de Productos Naturales Extremos, Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago 7800024, Chile.

J. Bórquez-Ramírez
Natural Products Laboratory. Chemistry Department. University of Antofagasta. Avenida Universidad de Chile # 028000. C.P. 1240000, Antofagasta, Chile.

R. Muñoz-Miranda
Natural Products Laboratory. Chemistry Department. University of Antofagasta. Avenida Universidad de Chile # 028000. C.P. 1240000, Antofagasta, Chile.

K. M. Rosales-Lopez
Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes, México.

E. Pérez-Molphe Balch
Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes, México.

Y. A. Gómez-Aguirre
Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes, México and CONACyT Research Fellow-Universidad Autónoma de Aguascalientes – Av. Universidad 940, Ciudad Universitaria, C.P. 20131, Aguascalientes, México.

F. Cruz-Sosa
Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa. Av. San Rafael Atlixco 186, Col. Vicentina C.P. 09340, Ciudad de México, México.

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