Organokinetics for Transient Cytomegalovirus Infection in Mice

The basic level of CMV diffusion into mouse organs was identified, as well as its relevance. This was conducted prior to our involvement in a larger project examining the immunological and molecular aspects of MCMV infection pathogenesis in Balb/c mice. We may have created potential to produce one or more vaccinations against the virus as a result of our endeavour. The virus was administered intraperitoneally to mice. The spleen, salivary glands, inguinal lymph nodes, liver, bone marrow, and lungs of mice were collected on days 1, 3, 7, 14, and 28 following infection. The organs were tested for MCMV-DNA expression, infectious viral titer, and morphological examination of a portion of the tissues that had been formalin-fixed and paraffin-embedded. Each organ had a unique configuration of the three components, which reflected the viral spread. Thus, substantial quantities of DNA and low titers of infectious virus were found in the lungs and bone marrow, indicating latency. High levels of MCMV-DNA and consistent titers of infective virus in the salivary glands indicated a chronic infection, as evidenced by high levels of MCMV-DNA and continuous titers of infective virus that remained beyond day 28. The most striking conclusion was that the latency detected in the spleen was most likely found in the stromal cells, not the lymphocytic component. We may have figured out the MCMV path in the mouse to this point.

Author (s) Details

Daniel Benharroch
Department of Pathology, Soroka Univ. Med. Ctr., and Faculty of Health Sciences, Ben Gurion Univ. of the Negev, Beer Sheva, Israel.

Bracha Rager
Department of Immunology, Microbiology and Genetics, Faculty of Health Sciences, Ben Gurion Univ. of the Negev, Beer Sheva, Israel.

Ory Ruvio
Division of Hematology, Soroka Univ. Med. Centr. and Faculty of Health Sciences, Ben Gurion Univ. of the Negev, Beer Sheva, Israel.

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