Development and Validation of UV-Spectrophotometric Method for the Determination of Lornoxicam in Microsponges

Objective: To develop and verify a new UV-spectrophotometric method for estimating the amount of lornoxicam in microsponges that is specific, precise, and simple. Lornoxicam is an oxicam-class non-steroidal anti-inflammatory medication (NSAID) with analgesic, anti-inflammatory, and antipyretic (fever-reducing) effects.

Thermo Scientific Evolution 201 UV-Vis spectrophotometer was used to make UV-spectrophotometric measurements with methanol as the medium. The absorption maximum (max) of the standard solution was determined by running its spectra from 200 to 400 nm. Lornoxicam has a maximum wavelength of 353 nm. The absorbance of standard solutions containing 3, 6, 9, 12, and 15 g/ml of drug solution was measured against a blank at an absorption maximum of 353 nm. The concentration was then plotted on the X-axis and the absorbance on the Y-axis, yielding a straight line. As per ICH recommendations, validation criteria such as linearity and range, selectivity and specificity, LOD and LOQ, accuracy, precision, and robustness were assessed.

With a correlation coefficient of 0.9995, the UV-spectrophotometric technique was found to be linear over a concentration range of 3.0-15.0 g/ml. Lornoxicam has a limit of detection (LOD) of 1.26 g/ml and a limit of quantification (LOQ) of 3.82 g/ml, respectively. The accuracy ranged from 99.21 to 99.60 percent. The percent RSD for repeatability, intraday precision, and interday precision was determined to be 0.473, 0.478-0.619, and 0.855-1.818, respectively. The UV technique proposed is shown to be reliable.

Conclusion: The suggested UV-Visible spectrophotometric method was verified using ICH recommendations, and the findings and statistical parameters showed that the developed method is sensitive, precise, reliable, and easy for estimating the amount of lornoxicam in microsponges.

Author (s) Details

Ayya Rajendra Prasad
Department of Pharmaceutics, Nirmala College of Pharmacy, Mangalagiri 522503, Andhra Pradesh, India.

Bannaravuri Thireesha
Department of Pharmaceutics, Nirmala College of Pharmacy, Mangalagiri 522503, Andhra Pradesh, India.

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