Experimental Evaluation of the Effect of Chloroform Inhalation as a Method of Euthanasia on the Cerebellum and Hippocampus of Adult Wistar Rats

In Nigeria, chloroform inhalation is a frequent method of rodent killing for study. The purpose of this study is to see how this form of sacrifice affects the cerebellum and hippocampus of Wistar rats. Twenty male Wistar rats, weighing 160 to 180 grammes, were separated into four groups, each with five rats. The control group, Group A, was sacrificed by cervical dislocation (a widely acceptable non-inhalation method of sacrifice). Groups B, C, and D were given 5 minutes of chloroform once a day (group B), twice a day for two days, and three times a day for three days. Four brains from each group were processed for antioxidant assays, and one brain from each group was preserved in Bouin’s fluid for histological examinations. Our findings reveal that inhaling chloroform had a dose-dependent negative impact on the antioxidant parameters investigated. That is, as the number of days increased, the negative effect became more pronounced. The histology findings confirmed this, with indications of cell necrosis in the cerebellum and hippocampus regions. This, too, demonstrated dosage dependence. As a result of our findings, we believe that chloroform inhalation is not the method of choice for rat death while investigating brain tissues or conducting brain-related research. As a result, chloroform sedation is not an appropriate method of rat sacrifice for experimental reasons, especially for investigating the rat brain.

Author (s) Details

Aguwa Ugochukwu Samuel
Anatomy Department, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

Okeke Somadina Nnamdi
Anatomy Department, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

Ezejindu Darmian Nnabuihe
Anatomy Department, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

Eze Chinyere Elizabeth
Anatomy Department, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

Azurunwa Ogechi
Anatomy Department, Faculty of Basic Medical Sciences, Madonna University Nigeria, Nigeria.

Obinwa Benedict Nzube
Anatomy Department, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

Ovie Ogbo. Felix
Anatomy Department, Faculty of Basic Medical Sciences, Madonna University Nigeria, Nigeria.

Obi Kelvin Chukwuemeka
Anatomy Department, Faculty of Basic Medical Sciences, University of Abuja, Nigeria.

Onwuelingo Sopuru
Anatomy Department, Faculty of Basic Medical Sciences, Madonna University Nigeria, Nigeria.

Okonkwo David
Anatomy Department, Faculty of Basic Medical Sciences, Madonna University Nigeria, Nigeria.

Doris Ogbuokiri
Anatomy Department, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

Okeke Chijioke
Anatomy Department, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria.

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