A Simple Electrochemical System to Evaluate Interactions between Bauhinia monandra Leaf Lectin (BmoLL) and Cratylia mollis Seed Lectin (Cramoll) Immobilized in Nafion Nanopores with Pseudomonas aeruginosa Endophytic Strain

The interaction of lectins immobilised in Nafion nanopores with Pseudomonas aeruginosa strains was studied using an electrochemical device. BmoLL, a leaf lectin specific for galactose obtained from Bauhinia monandra leaves, interacted with P. aeruginosa strain UFPEDA598, an endophytic bacterium isolated from B. monandra leaves. Cramoll is a seed lectin from Cratylia mollis that is selective for glucose-binding proteins. In the presence of galactose, a lectin inhibitor, the effect of this interaction was an increase in electrochemical potential and stability, with a binding preference for endophytic membrane carbohydrates. Cramoll had no interaction with the endophytic strain since the electrochemical potential did not differ from the control; nonetheless, UFPEDA-39 (IT2633); after the contact, a reduction in electrical potential was detected, with stability to the glucose-inhibited lectin. In order to recognise microorganisms in real time, we devised a simple, label-free approach. The biosensor was able to detect P. aeruginosa glycoconjugate surfaces from a variety of settings, making it helpful for selective identification.

Author (S) Details

Sandra R. Souza
Departamento de Educação, Universidade Federal Rural de Pernambuco, UFRPE, Rua Dom Manuel de Medeiros, s/n – Dois Irmãos, Recife, PE, CEP 52171-900, Brazil.

Benny F. Oliveira
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Jadilma C. Pimentel
Departamento de Educação, Universidade Federal Rural de Pernambuco, UFRPE, Rua Dom Manuel de Medeiros, s/n – Dois Irmãos, Recife, PE, CEP 52171-900, Brazil.

Sergio A. F. Ramos
Departamento de Educação, Universidade Federal Rural de Pernambuco, UFRPE, Rua Dom Manuel de Medeiros, s/n – Dois Irmãos, Recife, PE, CEP 52171-900, Brazil.

Janete M. Araújo
Departamento de Antibióticos, CB, UFPE. Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil.

Thiago H. Napoleão
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Patrícia Maria G. Paiva
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Maria T. S. Correia
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Luana Cassandra B. B. Coelho
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

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