Study on Isolation and Characterization of Crude oil Degrading Bacteria from Oil Spill Sites of Ennore Port

The goal of this study was to isolate and identify bacterial strains as well as assess their crude oil biodegradation capacity in vitro. Oil spills are a major cause of pollution and a constant threat to the maritime environment. Crude oil degrading microbes were isolated and identified using 16S rRNA gene sequencing to solve the problem. Under in vitro settings, a preliminary investigation was conducted to assess the potential of local bacterial strains to use crude oils as the sole carbon source. The oil breakdown efficiency of seven isolated bacterial strains from the oil spill impacted areas was tested. Using selected bacterial cultures, biodegradation studies were carried out in 1% crude oil for 12 days. Only three of the seven isolates, P. aeruginosa (AMETOS02), P. stuartii (AMETOS01 & AMETOS03), showed the highest oil degradation potential. P. stuartii (AMETOS03) shown potential biosurfactant activity, and the kind of hydrocarbon degraded was investigated further using gas chromatography–mass spectrometry (GC-MS). Our findings suggest that P. stuartii (AMETOS03) is a viable candidate for bioremediation of crude oil (hydrocarbon).

Author (S) Details

Gokulalakshmi Elayaperumal
Human Genetics Laboratory, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.

M. Vanitha Chinnathambi
Department of Biotechnology, Guru Nanak College Chennai, Tamil Nadu, India.

K. Ramalingam
Centre for Marine Exploration, Department of Marine Biotechnology, AMET University, Kanathur, Chennai-603112, India.

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