The Success of Good Old and Highly Specific Separation Method in the Isolation of Bioactive Proteins: From Bench to Bedside

By combining a large source of human proteins (500-fold concentrated human urine) with a highly selective isolation technology, ligand-affinity-chromatography, I was able to isolate not just soluble receptors, but also independent binding proteins and associated enzymes quickly and efficiently. The soluble forms of numerous receptors, as well as binding proteins, were isolated using this method: IL-6R, TNFRI, TNFRII, Type I Interferon receptor (IFN-(alpha)/(beta)R), Type II Interferon receptor (IFN-(gamma)R), IL-18 Binding Protein (IL-18BP), and IL-32 Binding Protein. These discoveries led to the hypothesis that soluble receptors and binding proteins are normal elements of bodily fluids in healthy people, but their amounts are modified in pathological conditions. Furthermore, the discovery of soluble receptors led to the discovery of their long-awaited cell surface ligand-binding counterparts. Several soluble proteins have been converted into medicines.

Author (S) Details

Daniela Novick
Molecular Genetics, The Weizmann Institute of Science, Israel.

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