The differential diagnosis of amyloid deposits detected by light, polarisation, and electron microscopy is the subject of this book.
The histologic, histochemical, immunohistochemical, and electron microscopic characteristics of the major forms of systemic and isolated amyloidosis are described.
Systemic amyloidosis is linked to the circulatory system, and the precursor spreads through the bloodstream; vessel walls are always involved, whether or not there is extravascular amyloid deposition.
Isolated amyloidosis is an extravascular phenomena that affects just one organ or tissue; its predecessors are not connected to the systemic circulation, and the vessel walls are spared; the process is isolated and does not involve blood vessels.
The most common kinds of systemic and localised amyloid deposits can be distinguished using basic immunohistochemical procedures.
Immunohistochemical examination of diverse amyloid deposits can be challenging, especially when the deposits are small and have a lot of background staining.
This book explains and illustrates histochemical methods for distinguishing between the most common systemic and isolated forms of amyloidosis.
In laboratories where electronmicroscopy, genomic DNA sequence analysis, or amino acid sequence identification techniques are not available, the histochemical procedures are recommended for widespread usage.
The current study and documentation are based on biopsy and autopsy material from the National Institute of Rheumatology’s Department of Pathology, as well as material sent in consultation throughout a thirty-year period.
The Appendix summarises the most important applicable methods. The methods used are simple to use and can be found in basic diagnostic facilities all around the world.
The findings are well-documented, including numbers and microphotographs to back them up.
All physicians and pathologists who work with or are interested in amyloidosis should read this book. The text is written in a way that both rheumatologists and general practitioners may understand.
Department of Pathology, Hospital of the Order of the Brothers of Saint John of God in Budapest, Hungary.
Department of Rheumatology, St. Margaret Clinic Budapest, Hungary.