Plant Regeneration in Indian Mustard [Brassica juncea (Linn.) Czern & Coss]: Experimental Investigation

Brassica juncea is an important mustard species that is farmed in India for edible oil. Using mature cotyledons and seeds as explants and embryogenic cell suspension cultures obtained from embryogenic friable calli, the current study establishes an efficient and repeatable plant regeneration process from callus. The best callus induction was achieved using MS basal media enhanced with 3.0 mgl-1 2, 4-D. Supplementing with 0.5 mgl-1 BAP and 0.5-1.0 mgl-1 2,4-D resulted in more regeneration via embryogenesis/organogenesis. It has been established that ripe seeds are preferable as explants, meaning that this explant has greater morphogenic potential. In addition, the genotype, type (s), relative concentrations, and combinations of plant growth regulators all had a significant impact on plantlet regeneration success. These strategies could be utilised in the future to change Indian mustard elite genotypes and select them in vitro.

Author(S) Details

N. Mishra
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

M. K. Tripathi
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

N. Tripathi
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

S. Tiwari
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

N. Gupta
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

A. Sharma
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.

M. K. Shrivastav
Department of Plant Breeding & Genetics, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

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