An Organic Solvent-free and Simple Procedure for Quantifying Tetracycline and 4-epi-tetracycline in Milk

This chapter offers a new quantification approach for an effective veterinary medicine (which is commonly used) that leads to improved food residue monitoring from the standpoint of assuring food safety. A quick, straightforward, small-scale sample preparation followed by an isocratic mobile phase HPLC for measuring tetracycline (TC) and its 4-epimer, 4-epi-tetracycline (4eTC) in cow’s milk was disclosed in this paper under organic solvent-free circumstances. Only homogenization using a handheld ultrasonic homogenizer and deproteinizing aqueous solution, followed by filtration with a disposable unit, was possible for sample preparation. An Inertsil WP300 C4 and isocratic 100 percent aqueous mobile with photodiode array detector were utilised to determine and identify the analyte. The data from the method validation met the worldwide analytical method acceptance requirements. The time it took to analyse one sample was less than seven minutes. No organic solvents were employed at any point during the procedure. The current method has the potential to be offered as an international harmonised analytical method for routine TC and 4eTC residue monitoring in cow’s milk.

Author(S) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan.

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