Evaluation and Pharmacological Screening of Endophytic fractions of Centella asiatica Linn Leaves for In-vitro Antioxidant Activity

We isolated fungal endophytes from Centella asiatica Linn leaves, then fermented and extracted the endophytes with non-polar solvents such as chloroform, ethyl acetate, and n-butanol. An invitro free radical scavenging activity experiment using reducing power, DPPH, and hydroxyl radical assays was followed by a preliminary phytochemical examination of endophytic crude extracts of leaves to evaluate the presence of primary and secondary metabolites. The chloroform fungal endophytic fractions were subjected to column chromatography using a gradient elution method in order to isolate a putative secondary metabolite. The reducing power of endophytic extracts of C. asiatica Leaf (CAL-1) (50-450g/ml) increased with increasing concentrations. The reaction of CAL-1 with DPPH radicals demonstrated good scavenging activity. 30.33 g/ml, 66.58 g/ml, 79.33 g/ml, and 96.39 g/ml were found to be the IC50 values for ascorbic acid, chloroform fraction, ethyl acetate, and n- butanol fraction, respectively. In the hydroxyl radical assay, the IC50 values for mannitol, chloroform fraction, ethyl acetate, and n-butanol fraction were determined to be 121.06 g/ml, 141.21 g/ml, 181.80 g/ml, and 189.90 g/ml, respectively. The antioxidant activity of endophytic crude ethyl acetate fractions was much higher than that of other fractions. As a result, ethyl acetate fungal endophytic fractions of Centella asiatica Linn leaves may be employed as an antioxidant to combat oxidative stress generated by free radicals. More research is needed to identify and describe the possible polyphenolic chemicals found in endophytic extracts, as well as their mode of action in the treatment of free radical-induced oxidative stress.

Author(S) Details

R. A. Shastry
Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad – 580002, Karnataka, India.

P. V. Habbu
Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad – 580002, Karnataka, India.

D. M. Smita
Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad – 580002, Karnataka, India.

Sudhir. R. Iliger
Department of Pharmaceutics, S.E.T’s College of Pharmacy, S.R. Nagar, Dharwad – 580002, Karnataka, India.

V. H. Kulkarni
Department of Pharmacology, S.E.T’s College of Pharmacy, S.R. Nagar, Dharwad – 580002, Karnataka, India.

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