Screening of N-Benzoyl Isoserine Methyl Ester (N-bime) for Anti-inflammatory Analgesic activity and Toxicity Profile in Animals: An Experimental Investigation | Chapter 2 | Research Aspects in Agriculture and Veterinary Science Vol. 5

Background: The basic mechanisms involved in the pathophysiology of many diseases are pain and inflammation. Rheumatic disorders are frequently treated with nonsteroidal anti-inflammatory medications (NSAIDs). The studied substance N-Benzoyl Isoserine Methyl Ester (N-bime) is a newly synthesised propionic acid derivative by National Chemical Laboratory, Pune. Because there is no biological data on this molecule, the current study will screen it for anti-inflammatory, analgesic, and toxicological profiles in animals.

The goal of this study was to screen N-bime for anti-inflammatory, analgesic, and anti-pyretic activity in animals, as well as to compare anti-inflammatory, analgesic, and anti-pyretic activity of N-bime to that of Ibuprofen, and to investigate its toxicity profile.

In rats, a single dosage toxicity investigation was conducted. Rat Hind Paw Oedema and Cotton Pellet Implantation were used to investigate anti-inflammatory activity. Acetic acid induced writhing and the Tail Pinch technique were employed to determine analgesic activity. Yeast induced Pyrexia was employed for evaluation of anti-pyretic efficacy. Ibuprofen was the positive control. Data are reported as mean±SEM. Statistical analysis was performed using analysis of variance and students unpaired ‘t’ test.

In the dose range of 1 mg – 500 mg / 100 gm body weight, the test chemical N-bime showed no evident detrimental effects or mortality in animals. It showed greater anti-inflammatory activities in higher doses as compared to Ibuprofen (p˂ 0.05). In acetic acid induced writhing test N-bime was found to be more effective than Ibuprofen in preventing writhes. In the Tail Pinch method, however, both failed to show analgesic efficacy. In the anti-pyretic test, N-bime demonstrated a progressive drop in temperature (P0.001).

Conclusions: The present investigation reveals that N-bime does possess anti- inflammatory, analgesic and modest anti-pyretic characteristics like the NSAIDs. It has demonstrated to be safe in the dose range of 1mg – 500 mg / 100 gm body weight in rats and mice.

Author(s) Details:

Maliha Niroomand,
Department of Pharmacology, B. J. G. M. C. and S. G. H., Pune, Maharashtra, India.

Kalpana U. Shah,
Department of Pharmacology, B. J. G. M. C. and S. G. H., Pune, Maharashtra, India.

Balasaheb B. Ghongane,
Department of Pharmacology, B. J. G. M. C. and S. G. H., Pune, Maharashtra, India.

Please see the link here: https://stm.bookpi.org/RAAVS-V5/article/view/5711