To avoid residual chemicals in seafoods, it is necessary to improve residue monitoring in these foods, which necessitates the development of an accurate and efficient quantitative approach for each target chemical substance. An inexpensive, simple, rapid, and microscale sample preparation technique is described in this chapter, followed by a high-performance liquid chromatography (HPLC) coupled photodiode array (PDA) detector for simultaneous quantification of malachite green (MG) and its metabolite, leucomalachite green (LMG) in cultured eel. The HPLC-PDA was performed on a C4 column with an isocratic mobile phase. Analytes were removed from the sample using a handheld ultrasonic homogenizer, and the sample was purified using MonoSpin® C18 centrifugal monolithic SPE spin mini-columns. Analyses of spiked eel samples have shown that the recommended technique works, with recoveries > 95%, relative standard deviations 2%, and total analytical time 15 minutes per sample (24 samples 4 hours).
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka 558-8585, Japan.
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