Investigating the Effect of Storage and Drying Conditions on DNA Extracted from D. brandisii
In India, Dendrocalamus brandisii is considered one of the most valuable multifunctional bamboo species. For PCR amplification and DNA fingerprinting study of plant species, pure genomic DNA isolation is essential. Without using liquid nitrogen, DNA was extracted using the CTAB technique with minor modifications. The influence of storage and drying conditions on DNA quality and quantity was investigated. Freshly collected leaf samples were held at 4°C for varying periods of time (10 days, 30 days, and 60 days) before genomic DNA was extracted. The quantity and purity of the DNA recovered were determined using spectrophotometry and agarose gel electrophoresis. The average yield per 50 mg was 25.3 to 337.32 ng DNA. When comparing fresh leaves preserved at different intervals to silica dried leaves, the mean yield and purity of the recovered DNA was higher. Without the use of PCR enhancers, DNA was further diluted and optimised for PCR amplification with universal primers rbcL 1F & 724R and rbcL aF & aR. The improved approach described above has shown to be beneficial since it is simple, efficient, and uses inexpensive reagents to produce high-quality DNA from D. brandisii leaves.
Author(S) Details
Tresa Hamalton
Institute of Wood Science and Technology (ICFRE), Bangalore, India.
V. Lavanya
Institute of Wood Science and Technology (ICFRE), Bangalore, India.
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