Bio-Monitoring and Occupational Risk Assessment in Human Population from Nuclear Research Centre – using Exfoliated Buccal Epithelial Cells and Peripheral Blood Lymphocytes

The goal of the study is to evaluate the baseline frequency of micronuclei in exfoliated buccal epithelial cells (BE cells) and peripheral blood lymphocytes from the same people in a randomly selected human population of a nuclear research facility. A non-invasive method to assess radiation and mutagen exposure is urgently needed. Our nuclear research center’s monitoring personnel evaluated the buccal epithelial micronuclei test for this reason when they were there for their yearly physicals. The micronucleus test is a sensitive indicator that counts the number of broken or lost chromosomes. To assess the baseline micronuclei frequency in exfoliated buccal epithelial cells and lymphocyte micronuclei, a study was conducted on 50 randomly selected persons working in nuclear research centres. test for buccal micronucleus cytome On slides stained with Geimsa’s dye, the blood lymphocyte cytokinesis blocked micronuclei assay (CBMN) and BMNcyt were carried out. We provide the average frequency of spontaneous micronuclei in blood lymphocytes and buccal epithelial cells. The average number of micronuclei (MN) found in buccal epithelial cells of men and females was 0.49 and 0.10, respectively, with a range of 0.09 to 2.92. (range 0.00 to 2.06). MN frequency was 0.66 0.10 in buccal epithelial cells (range 0.00 to 2.92). The MN frequencies were determined to be 8.61 0.10 (range 3.00 to 17.00) in females and 9.15 1.00 (range 3.00 to 22.00) in men in human peripheral blood cells from the same donors. In cytokinesis-blocked lymphocytes, the overall MN frequency was 8.96 0.73 Bi-nucleated (BN) cells (Range: 3.00 to 22.00). Human peripheral blood lymphocytes and buccal epithelial cells both have spontaneous micronuclei at a baseline frequency that is correlated. As a result, buccal epithelial cell micronuclei provide for an effective noninvasive biomonitoring technique.

Author(s) Details:

N. K. Sharma,
Low Level Radiation Research Section, Radiation Biology and Health Sciences Division, Biosciences Group, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India

S. D. Kamble,
Pathology Laboratory, Occupational Health Centre, Medical Division, Modular Laboratory, Bhabha Atomic Research Centre, Trombay Mumbai 400085, India.

D. Jeyaprakash,
Occupational Health Centre, Medical Division, Modular Laboratory, Bhabha Atomic Research Centre, Trombay Mumbai 400085, India.


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Keywords: Exfoliative buccal mucosa cells, exfoliative cytology, buccal micronucleus cytome, buccal epithelial cells, cytokinesis blocked micronucleus assay, CBMN

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