In vitro Tuberization of Glory Lily (Gloriosa superba. L): Experimental Investigation

Gloriosa superba L. (Colchicaceae) usually known as Kalahari and Glory lily was an export familiarize medicinal plant. Colchicine was the bigger tropalone alkaloids of medicinal importance that cures gout, tumor, rheumatism. Commercially propagated by tubers which are ‘V’ or ‘L’ formed, but was considered slow with weak multiplication percentage of 1:1 every year. The present study has described a doable procedure for artificial tuberization employing non-dormant tubers of Gloriosa superba L. improved with variable quantities of auxin, cytokinin, and gibberellins on Murashige and Skoog medium. When sterilized with 70% flammable liquid for 30 seconds and then 60 seconds in HgCl2, sprouted tuber bud explants had a contamination portion of only 8.00%. The maximum response for primary vegetable (100%) and secondary vegetable (100%) development was observed in MS medium complemented with 4.0 mgl-1 BAP and 1.0 mgl-1 NAA. This also allowed the most tubers (1.77) from a sole explant. GA3 (1.0 mgl-1) was found to be essential for dash elongation, inasmuch as IAA (1.0 mgl-1) in combination with IBA (0.5 mgl-1) was productive for root induction on MS medium.
Author(s) Details:

Anandhi Selvarasu,
SRM College of Agricultural Sciences, Vendhar Nagar, Baburayanpettai, Acharapakkam-603 203; Chengalpattu (Dist.), India.

Rajamani Kandhasamy,
Department of Floriculture and Landscape Architecture, Horticultural College & Research Institute, Tamil Nadu Agricultural University, Coimbatore – 641 003, Tamil Nadu, India.

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Keywords: Glory lily, MS medium, tuber node, shooting, rooting, primary tubers

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