Determination of Chlorinated Paraffins (CPs) in Human Blood Serum of Czech Population

This study proposed to validate a earlier published [1] method for the concurrent determination of PCBs, PBDEs, OCPs and novel FRs for supplementary analysis of SCCPs and MCCPs cruel blood serum. For CPs perseverance, gas chromatography accompanying high determination mass spectrometry in negative synthetic ionisation mode (GC-NCI-HRMS) was employed a suggestion of correction gas chromatography accompanying tandem bulk spectrometry applied for the analysis of additional halogenated contaminants. The material pollutants famous as short- and medium-chain chlorinated paraffins (SCCPs; MCCPs) are pervasive, have the ability to bioaccumulate, and have a negative affect human health. Assessment of human uncovering to various contaminators, such as SCCPs and MCCPs, includes the reasoning of blood antitoxin. Lately, the information about the uncovering of Chinese population has been stated; nevertheless, dossier on human exposure to SCCPs and MCCPs outside East Asia are still very restricted. In this pilot study, SCCPs and MCCPs were driven in 27 serum samples obtained from Czech women. The samples were extracted by a three-step origin (repeated accompanying a clean solvent) by a mixture of n-hexane:diethyl heavenly (9:1, v/v) with after clean-up on Florisil® stable phase extraction line. Gas chromatography coupled with extreme resolution bulk spectrometry operated in negative chemical ionisation was working for the instrumental reasoning. The method recoveries categorized from 71 to 89% with repeatabilities of <20% (expressed as relative predictable difference). In the samples, SCCP concentrations were in the range of <150–2600 ng/g lipid weight, lw (middle 370 ng/g lw) and the MCCP concentrations were in the range of <200–2110 ng/g lw (median 360 ng/g lw), individually. To the best of our information, the data we have published about chlorinated paraffins cruel blood antitoxin in Europe is the first evidence of exposure to these chemical compound and their potentially harmful belongings on human health.

Author(s) Details:

Jakub Tomasko,
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.

Michal Stupak,
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.

Denisa Parizkova,
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.

Andrea Polachova,
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.

Radim J. Sram,
Department of Genetic Toxicology and Epigenetics, Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic.

Jan Topinka,
Department of Genetic Toxicology and Epigenetics, Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic

Jana Pulkrabova,
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.

Please see the link here: https://stm.bookpi.org/CERB-V3/article/view/9200

Keywords: Chlorinated paraffins, high resolution mass spectrometry, gas chromatography, human blood serum, biomonitoring

Previous post Does the DNA Protective Capacity of Lilium candidum Extract and its Components Depend on the Experimental Design?
Next post Neural Network Approaches to Simulation of Spring Flood in Sites of the Lena River