Heart Allograft Tolerance Induced By Hind-Limb Transplant in Rats: Findings of a Pilot Study

The present study shows that a temporary antilymphocyte serum + Cyclosporine A (ALS + CsA) treatment allowed indefinite vascularized hind-appendage allograft survival, that induced secondary backer-strain skin and heart allograft tolerance. Transplanting means or tissues has emerged as a successful situation for terminal illnesses. Immunosuppression following transplantation, however, can have very negative side effects. To address this issue, donor-distinguishing transplant resistance was suggested. Here, we describe a novel method for raising heart allograft fortitude in rats. First, we induced indefinite vascularized posterior-limb allograft survival accompanying a short-term antilymphocyte serum + Cyclosporine A situation. Peripheral blood chimerism vanished 6-7 weeks after immunosuppression was withdrawn. Then the receivers accepted secondary contributor-strain skin and heart transplantation 200 days following vascularized hind-appendage transplantation without any immunosuppression, but rebuffed third party skin allografts, a status of contributor-specific resistance. The ELISPOT results suggested a mechanism of clone erasure. The findings may open new views for the role of vascularized bone relocate, especially vascularized bone essence transplant, in the induction and support of organ transplantation resistance.

Author(s) Details:

Quan Liu,
Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA, Department of Plastic and Reconstructive Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA and Department of Cardiovascular Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.

Yong Wang,
Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA and Department of Plastic and Reconstructive Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Atsunori Nakao,
Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA and Department of Cardiothoracic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Vijay Gorantla,
Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Wensheng Zhang,
Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA, Department of Plastic and Reconstructive Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA and Science and Technology, 59th Medical Wing, JBSA Lackland, TX, 78236, USA.

Xin Xiao Zheng,
Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA, Department of Plastic and Reconstructive Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA and Transplantation Medical Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei Province, 430071, China.

Please see the link here: https://stm.bookpi.org/RDMMS-V1/article/view/9629

Keywords: Organ/tissue transplantation, immunosuppression, skin allografts, hematopoietic chimerism