Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes).
A murine Associate in Nursingtibody|antibody} specific for calf enteral alcalescent enzyme has been ready and utilized in an unlabelled protein bridge technique for labeling being antibodies. This procedure–the alcalescent enzyme being anti-alkaline enzyme (APAAP) method–gives glorious immunocytochemical labeling of tissue sections and cell smears, comparable in clarity and intensity thereto achieved with immunoperoxidase labeling. If the catalyst label is developed with a phenol salt as a coupling agent and quick Red or hexazotized new fuchsin as a capture agent, a vivid red reaction product is obtained that is incredibly simply detected by the human eye. 
A method for the rapid determination of alkaline phosphates with five cubic millimeters of serum.
The alkalic enzyme of the humour will increase early and markedly in rachitis and returns fully to traditional solely when healing is complete. attributable to this reality, humour enzyme is that the most satisfactory index currently legendary for the detection of this deficiency. The enzyme activity of humour isn’t strictly specific during this respect and has conjointly well-tried clinically helpful in an exceedingly variety of different pathological states; e.g., Paget’s sickness, glandular disorder, disease, etc. 
A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots
A rapid, sensitive methodology has been developed to discover antibody-antigen complexes on “Western blots.” The ways of H. Towbin, T. Staehlin, and J. Gordon were wont to separate and blot the antigens onto nitrocotton. The remaining sites of attachment were blocked and also the nitrocotton was washed with polyoxyethylenesorbitan monolaurate (Tween 20). The blot was then reacted with the serum or vegetative cell supernate to be tested. once the antigen-antibody reaction was completed, the blot was washed and treated with anti-antibody that has been conjugated to alkaline enzyme. 
Relation of placental alkaline phosphatase expression in human term placenta with maternal and offspring fat mass
Introduction: alkalic enzyme is concerned in enteric lipoid transport and within the development of fat. Placental alkalic enzyme is localised to the microvillous cell membrane of the placental syncytiotrophoblast at the maternal–fetal interface, however its role is unclear. we tend to investigated the relations of placental alkalic enzyme activity and ribonucleic acid expression with maternal body composition and offspring fat mass in humans. 
Study of Prostate Specific Antigen, Alkaline Phosphatase and Vitamin B12 in Prostate Cancer
Objective: This study aimed to seek out the relative risks of glandular cancer incidence related to screening by humour prostate-specific matter (PSA), base-forming enzyme (ALP) and B complex levels.
Methods: humour samples of 488 subjects on top of forty years while not treatment or puncture/rectal examination were screened for PSA levels mistreatment luminescence. glandular cancer cases and management teams were analysed for B complex mistreatment luminescence and base-forming enzyme using absolutely machine-controlled chemistry instrument. The relation of mount and B complex to levels of PSA in glandular cancer was examined. 
 Cordell, J.L., Falini, B.R.U.N.A.N.G.E.L.O., Erber, W.N., Ghosh, A.K., Abdulaziz, Z., MacDonald, S., Pulford, K.A., Stein, H.A.R.A.L.D. and Mason, D.Y., 1984. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). Journal of Histochemistry & Cytochemistry, 32(2), pp.219-229. (Web Link)
 Otto, A., Oliver, H. and Jane, M., 1946. A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. Journal of Biological Chemistry, 164, pp.321-329. (Web Link)
 Blake, M.S., Johnston, K.H., Russell-Jones, G.J. and Gotschlich, E.C., 1984. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Analytical biochemistry, 136(1), pp.175-179. (Web Link)
 Relation of placental alkaline phosphatase expression in human term placenta with maternal and offspring fat mass
Birgit Hirschmugl, Sarah Crozier, Nina Matthews, Eva Kitzinger, Ingeborg Klymiuk, Hazel M Inskip, Nicholas C Harvey, Cyrus Cooper, Colin P Sibley, Jocelyn Glazier, Christian Wadsack, Keith M Godfrey, Gernot Desoye & Rohan M Lewis
International Journal of Obesityvolume 42, pages1202–1210 (2018) (Web Link)
 V. Bhivapure, R., M. Dalvi, S., Yeram, N., W. Patil, V. and G. Abichandani, L. (2018) “Study of Prostate Specific Antigen, Alkaline Phosphatase and Vitamin B12 in Prostate Cancer”, International Journal of Biochemistry Research & Review, 22(2), pp. 1-7. doi: 10.9734/IJBCRR/2018/42394. (Web Link)