Under UV irradiation to irritate active oxygen from the agents, the antibacterial efficacy of the photocatalysts TiO2, TiO2+CuO, and WO3+CuO was tested against E. coli, K. pneumoniae, MRSA, and P. aeruginosa. Drawing a bacterial growth curve, colony counting on a cultivated Petri dish, calculating generation time in liquid medium, and evaluating the bacteria’s survival rate using Phase One Plus Realtime PCR were among the analytical criteria. The most effective growth inhibitor was tungsten oxide co-doped with copper (WO3+CuO), followed by TiO2+CuO and TiO2 alone. WO3+CuO had the highest bacterial killing potency (survival rate) of the agents studied, whereas co-doped TiO2+CuO and TiO2 alone had lower activity than WO3+CuO. At 3 hours incubation, photocatalyst-exposed bacteria showed unusual growth curve behaviour, such as re-growth after growth inhibition. K. pneumoniae appeared to be immune to the effect of photocatalysts on growth, indicating the emergence of radical oxygen resistant bacteria. To prevent the emergence of active oxygen resistant bacteria, a more thorough review should be conducted to explain this undesirable phenomenon.
Author (s) Details
Yamanouchi Kanako
Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, 66-1 Honcho, Hirosaki 036-8564, Japan.
Satoh Yui
Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, 66-1 Honcho, Hirosaki 036-8564, Japan.
Ichinose Momo
Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, 66-1 Honcho, Hirosaki 036-8564, Japan.
Tsujiguchi Takakiyo
Department of Radiological Technology, University Graduate School of Health Sciences, 66-1 Honcho, Hirosaki 036-8564 Japan.
Satoh Toshikazu
Izumi Giken Co. Ltd. 456-5 Yatogo, Kumagaya 360-0205 Japan.
Sasaki Jin-ichi
Department of Nursing Science, Aomori University of Health and Welfare, 58-1 Mase Hamadate, Aomori 030-8505, Japan.
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