Press Release on Agglutinin Research: April – 2019

Tumor recognition of peanut agglutinin-immobilized fluorescent nanospheres in biopsied human tissues

We are work associate imaging agent for early detection of large intestine cancer. The agent, named the nanobeacon, is coumarin 6-encapsulated polystyrenenanospheres whose surfaces are lined with poly(N-vinylacetamide) and peanut antibody that reduces non-specific interactions with the conventional tissue layer and exhibits high affinity for terminal sugars of the Thomsen-Friedenreich matter, that is expressed cancer-specifically on the tissue layer, severally. we tend to expect that cancer is diagnosed by sleuthing illuminationof intracolonically administered nanobeacon on the tissue layer surface. within the gift study, biopsied human tissues were accustomed judge the potential use of the nanobeacon within the clinic. before the clinical study, diagnostic capabilities of the nanobeacon for detection of large intestine cancer were valid exploitation twenty production batches whose characteristics were fine-tuned with chemicals for the aim. Ex vivo imaging studies on sixty six traditional and sixty nine cancer tissues faraway from the colons of normal and orthotopic mouse models of human large intestine cancer, severally, incontestable  that the nanobeacon detected large intestine cancer with glorious capabilities whose rates of true and false positives were ninety one and fifth, severally. within the clinical study, traditional and tumour tissues on the massive viscus tissue layer were biopsied endoscopically from eleven patients with large intestine tumors. histologic analysis disclosed that nine patients suffered from cancer and therefore the rest had benign tumor. Mean visible radiation intensities of tumour tissues treated with the nanobeacon were considerably more than those of the corresponding traditional tissues. Correlation of relation of the intensity in people was discovered in cancer patients with a high chance (89%); but, the chance reduced to fiftieth in benign tumor patients. There was an affordable probability for diagnosing of large intestine cancer by the nanobeacon applied to the tissue layer of the massive intestine. [1]

Splenic Marginal Zone B-Cell Lymphoma With Splenic Infarction in a Patient With Cold Agglutinin Disease.

We report a case of splenetic marginal zone B-cell malignant neoplastic {disease} discovered during a 73-year-old man with cold antibody disease. PET/CT disclosed hypertrophy with focally intense uptake of F-FDG and diffusely inflated bone marrow uptake, that was thought-about to be secondary to anemia. excision was performed. The histopathology of the spleen showed splenetic marginal zone B-cell malignant neoplastic disease with partial splenic pathology, that correlative with the world of focal intense FDG uptake. reckoning on the time since onset, splenetic infarctions will gift as focal FDG accumulation. [2]

Bacterial expression, purification and biophysical characterization of wheat germ agglutinin and its four hevein‐like domains

Wheat germ antibody (WGA), a polyose binding glycoprotein, has attracted increasing interest thanks to its distinctive characteristics like conformational stability, binding specificity and transcytosis capability. To pave the method for the study of the molecular basis of WGA’s structural stability and binding capability, likewise on facilitate its use in medical specialty and biotechnological developments, we have a tendency to created recombinant WGA and its four isolated hevein‐like domains during a microorganism system. All the proteins were expressed as fusion constructs coupled to a thioredoxin domain, that was enzymatically or with chemicals discharged. The structural and ligand‐binding properties of recombinant WGA were kind of like the wild glycoprotein. The four isolated domains doubled and were ligand‐binding competent, indicating that every domain constitutes Associate in Nursing freelance folding unity. The biophysical characterization of the recombinant domains sheds new lightweight on the tortuous folding and binding behavior of this emblematic glycoprotein. [3]

Production of Cold Agglutinins in Rabbits immunized with Human Erythrocytes treated with Mycoplasma pneumoniae

The reaction product of I substance with eubacteria pneumoniae, instead of M. pneumoniae alone, is also answerable for the assembly of cold antibody in man. [4]

Seroprevalence of Salmonella Agglutinins among Apparently Healthy Students of a Tertiary Institution in North-Eastern Nigeria

Aim: To determine the level of Salmonella agglutinin and its titre among apparently healthy students of Tertiary Institution in North-eastern Nigeria.

Study Design: A cross-sectional study on Seroprevalence of Salmonellaagglutinin;

Place and Duration of the Study: Department of Biological Science Technology, Federal Polytechnic Mubi, Adamawa State, between September to December, 2016.

Methodology: This study was a cross-sectional study in which 200 apparently healthy  students of Federal Polytechnic Mubi were tested for Salmonella agglutinin using both slide and tube agglutination methods.

Statistical Analysis: Non-parametric Mann-Whitney statistics and least significance difference (LSD) were used to test for significant difference in all the data obtained. Significance difference was taken when P˂0.05.

Results: The result showed that the number of male with positive Widal agglutinin was significantly higher than that of female (P=0.028). The number of positive titre was significantly higher in age group 21-25 years (P=0.002) when compared to other age brackets. However, there was no statistical difference between the age bracket 16-20 years and 31-35 years (P=0.747). The result of the agglutination titre showed that more male had Salmonellatitre for Salmonella Typhi O (81.8%) and Salmonella Typhi H (72.7%) while more female had Salmonella agglutinin titre for Salmonella Typhi H (44.4%) and Salmonella Paratyphi B-H (33.3%). It was also observed that only agglutinin for Salmonella Paratyphi A-O, B-O, C-O, B-H and C-H were present in the sera of male subjects up to the titre of 160 and at frequencies ranging from 1.6-58.8%. Furthermore, among females, it was observed that only agglutinins for Salmonella Paratyphi A-O, Salmonella Typhi C-H and Salmonella Typhi O and H were present in the sera of the subjects up to the titre of 160 and at frequencies ranging from 2.5-25.0%.

Conclusion: Detecting Salmonella agglutinin titre up to 160 among apparently healthy individuals is of public health significance. Thus, there is need to monitor this trend and create awareness on environmental and behavioural risk factors for Salmonella infections. [5]

 Reference

[1] Kumagai, H., Yamada, K., Nakai, K., Kitamura, T., Mohri, K., Ukawa, M., Tomono, T., Eguchi, T., Yoshizaki, T., Fukuchi, T. and Yoshino, T., 2019. Tumor recognition of peanut agglutinin-immobilized fluorescent nanospheres in biopsied human tissues. European Journal of Pharmaceutics and Biopharmaceutics136, pp.29-37. (Web Link)

[2] Nakamoto, R., Okuyama, C., Utsumi, T. and Yamamoto, Y., 2019. Splenic Marginal Zone B-Cell Lymphoma With Splenic Infarction in a Patient With Cold Agglutinin Disease. Clinical nuclear medicine. (Web Link)

[3] Leyva, E., Medrano‐Cerano, J.L., Cano‐Sánchez, P., López‐González, I., Gómez‐Velasco, H., del Río‐Portilla, F. and García‐Hernández, E., 2019. Bacterial expression, purification and biophysical characterization of wheat germ agglutinin and its four hevein‐like domains. Biopolymers, p.e23242. (Web Link)

[4] Production of Cold Agglutinins in Rabbits immunized with Human Erythrocytes treated with Mycoplasma pneumonia

TEN FEIZI, D. TAYLOR-ROBINSON, M. D. SHIELDS & R. A. CARTER

Nature volume 222, pages 1253–1256 (1969) (Web Link)

[5]Tula, M., Iyoha, O., O. Okojie, R., Filgona, J. and A. Onyeje, G. (2018) “Seroprevalence of Salmonella Agglutinins among Apparently Healthy Students of a Tertiary Institution in North-Eastern Nigeria”, International Blood Research & Reviews, 8(3), pp. 1-7. doi: 10.9734/IBRR/2018/43362.(Web Link)

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