Introduction: The aims of the research were to carry out a clinical trial of ethno-veterinary medicinal products for mastitis, to carry out an intervention effect review on the reduction of antibiotic residues in milk when herbal formulations are used, and to determine in vitro the pharmacological understanding of the ingredients in the formulations based on published literature and phytochemical evaluation. Methods: Mastitis herbal formulations have been reported by a healer, and protection and efficacy have been verified using the technique previously suggested[1]. The California Mastitis Test (CMT) approach has established clinical mastitis. Before and after treatment with Ethno-Veterinary Medicine (EVM), pH, Somatic Cell Count (SCC) and Electric Conductivity (EC) were controlled and reported in field studies involving 27 animals. For 7 days, freshly prepared fine herbal paste was applied externally over the udder 10 times a day. The udder was well washed with water prior to each application and the milk from all quarters was fully removed. Two lemon fruits were also fed twice daily to the affected cows. Before and after intervention, bulk milk samples were checked for the presence of antibiotic residue(s) in milk. Based on the literature and phytochemical examination, the pharmacological understanding of the ingredients in the formulation was evaluated. Results: In animals with mastitis, the select parameters (pH, SCC, EC) became standard within 6-7 days of treatment. The development of milk returned to near normal at the stage of pre-mastitis. Thus, with this formulation, mastitis can be treated effectively. The intervention effect review showed a reduction of antibiotic residues in milk by 18 to 49 percent. The [Aloe vera (L.) Burm Mix. F, Longa Curcuma L. Calcium hydroxide] works synergistically and, due to its wide range of antimicrobial, anti-inflammatory and immunomodulatory activities, offers an effective treatment for clinical mastitis. Conclusion: With this formulation, mastitis can be controlled effectively. The intervention effect study showed a reduction of antibiotic residues in milk by 18-49 percent. The [Aloe vera (L.) Burm Mix. F, Longa Curcuma L. Calcium hydroxide] works synergistically and, because of its wide range of antimicrobial, anti-inflammatory and immunomodulatory activities, offers an effective treatment for clinical mastitis. In the treatment of mastitis, this conventional formulation is very effective. The intervention impact analysis showed a decrease in antibiotic residues in milk of 18 to 49 percent, suggesting that natural products based on EVM are an important alternative to synthetic chemicals in the treatment of cattle mastitis.
Author(s) Details
M.N. Balakrishnan Nair
TransDisciplinary University, School of Health Sciences, Veterinary Ayurveda group, Bangalore-560106, Karnataka, India.
N.Punniamurthy
EVM Herbal Training and Research Centre, Tamil Nadu Veterinary and Animal Sciences University, Thanjavur-613403, India.
P.Mekala
EVM Herbal Training and Research Centre, Tamil Nadu Veterinary and Animal Sciences University, Thanjavur-613403, India.
N.Ramakrishnan
EVM Herbal Training and Research Centre, Tamil Nadu Veterinary and Animal Sciences University, Thanjavur-613403, India.
S. K. Kumar
TransDisciplinary University, School of Health Sciences, Veterinary Ayurveda group, Bangalore-560106, Karnataka, India.
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