Single Step Aqueous Two-Phase System (ATPS), for Purification of Glucose Oxidase (GOx)

Posted on January 4, 2021January 4, 2021Categories BiologyTags , ,   Leave a comment on Single Step Aqueous Two-Phase System (ATPS), for Purification of Glucose Oxidase (GOx)

Glucose oxidase is an intracellular and extracellular enzyme of the genus Aspergillus and Penicillium, developed on an industrial scale. The purpose of this research was to study the isolation of Aspergillus niger glucose oxidase (GOx) in an aqueous two-phase system consisting of PEG 7500 (150 g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), divided into polymer phase enzymes. GOx (69.2 percent) was recovered by sequential extraction by 11.8 fold purification in the polymer process, giving … Continue reading “Single Step Aqueous Two-Phase System (ATPS), for Purification of Glucose Oxidase (GOx)”

Purification and Characterization of Cellulase from Aspergillus niger

Posted on October 17, 2020Categories Microbiology and BiotechnologyTags , , ,   Leave a comment on Purification and Characterization of Cellulase from Aspergillus niger

Microbial enzyme is an important biological substance or biological molecules that are tested by living organisms that act as biocatalyst to bring out a specific biochemical reaction. Enzymes are biochemical catalysts that carry out chemical reaction to speed up the biological. Chemical activity inside or outside the cell. These are referred as “Biocatalyst”. An extracellular cellulose producing Aspergillus niger isolated from the forest soil of Courtalam Taluk, Tirunelveli District. Based on the biochemical test and 18S rRNA gene sequencing the … Continue reading “Purification and Characterization of Cellulase from Aspergillus niger”