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A Review on Innovating the Concept and Practice of Two-Dimensional Gel Electrophoresis in the Analysis of Proteomes at the Proteoform Level

Editor JournalGazett February 17, 2021February 17, 2021Chemistry and Biochemistrybottom-up proteomics, isoelectric focusing, iTRAQ, liquid chromatography, mass spectrometry, post-translational modification, protein speciation, protein species, proteoform, SILAC, splicing, TMT, top-down proteomics, Two-dimensional gel electrophoresis Leave a comment

A significant and well-established technological platform allowing comprehensive top-down proteomic analysis is the two-dimensional gel electrophoresis (2DE). However, 2DE’s long-held but now increasingly obsolete traditional principles have clearly impacted its application at the level of protein species/proteoforms to in-depth investigations of proteomes. It is time for a new definition of 2DE for proteomics to be popularized. Any provided “protein” is now recognized to consist of a series of proteoforms with the production and enrichment of the proteome concept. Thus, it … Continue reading “A Review on Innovating the Concept and Practice of Two-Dimensional Gel Electrophoresis in the Analysis of Proteomes at the Proteoform Level”