Study on Cloning, Sequencing and Phylogenetic Analysis of Granulocyte Macrophage Colony Stimulating Factor of Indian Cattle (Bos indicus)

The cDNA coding for Granulocyte macrophage colony stimulating factor (GM-CSF) from LPS activated macrophages was amplified using reverse transcriptase polymerase chain reaction (RT-PCR), which was then cloned into the pCNDA vector and sequenced. The amplified bovine GM-CSF cDNA was 755

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