Study on Cloning, Sequencing and Phylogenetic Analysis of Granulocyte Macrophage Colony Stimulating Factor of Indian Cattle (Bos indicus)

The cDNA coding for Granulocyte macrophage colony stimulating factor (GM-CSF) from LPS activated macrophages was amplified using reverse transcriptase polymerase chain reaction (RT-PCR), which was then cloned into the pCNDA vector and sequenced. The amplified bovine GM-CSF cDNA was 755

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Analysis of Genetic Diversity in Chickpea Employing ISSR Markers

To detect genetic variations, inter simple sequence repeat (ISSR) markers are commonly utilised. ISSR markers were used to determine genetic diversity in 13 chickpea accessions, both cultivated and wild. The pentanucleotide repeat primer UBC-879 generated the best amplification patterns of

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New Tool for Phylogenetic Analysis of Helicobacter pylori: An Advanced Study

The goal of this study was to find canonical insertion/deletion (INDEL) markers in the Helicobacter pylori genome and develop an INDEL-typing approach for strain classification. A local database of nucleotide sequences of 69 H. pylori strains was established for comparative

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