Study on Cloning, Sequencing and Phylogenetic Analysis of Granulocyte Macrophage Colony Stimulating Factor of Indian Cattle (Bos indicus)

The cDNA coding for Granulocyte macrophage colony stimulating factor (GM-CSF) from LPS activated macrophages was amplified using reverse transcriptase polymerase chain reaction (RT-PCR), which was then cloned into the pCNDA vector and sequenced. The amplified bovine GM-CSF cDNA was 755

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Optimizing in vitro Generation of Interleukin-10 Secreting B Regulatory Cells

Bregulatory cells are a subpopulation of IL-10-secreting B-cells that are frequently identified as CD19+/38hi/24hi (B-regs). Changes in stimulating molecule concentration have an influence on the population of human Interleukin-10 secreting B-regs. For possible cell treatment, B-regs can be generated by

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